INFLUENCED OF ORGANIC WASTE ON PHYSICOCHEMICAL PROPERTIES OF SPENT ENGINE OIL CONTAMINATED SOIL SUPPLEMENTED WITH FUNGI ISOLATED FROM MECHANIC WORKSHOPS IN MINNA, NIGERIA

Abstract

Samples of spent engine oil the contaminated soils were collected from Shanchaga, Maikunkele, Shiroro, Tungas and Bosso mechanic workshops located in Minna, Nigeria. Four samples of o.5kg each per location were collected making a total of 2kg of soil sample per site and 10kg from the five sites. Non-oil Contaminated soil samples were collected from m Biological Garden. of the Department of Plant Biology, Federal University of Technology, Minna, Nigeria. The fungi were isolated from the mechanic workshop soils using dilution plate method in mineral salt medium. The influenced of spent engine oil on soil properties supplemented with fungi was investigated five treatments (25g/kg50g/kg, 75g/kg, 100g/kg and 125g/kg) of different weights of COW dung were applied to the soil in the pots measuring 15cm X 35cm filled with one kilogram (1kg) of steam sterilized soil. Standard Suspension (10.02 X 10 cfu/ml) of each of the pure fungal isolate the from obtained mechanic workshop soils were added to the pots mixed and thoroughly with each of the 5ml of oil engine spent contaminated soil. The contaminated soils were allowed to stand for one week before the application of the different treatments. All treatments were replicated three times and the contents of each pot were watered and tilled twice a week for aeration. The results of the analysis of the soil supplemented with Rhizopus stolonifer revealed that spent engine oil had no effect on the silt, sand and clay properties in all the soils. The increased in concentration of the organic carbon as a results of spent engine oil contamination was significantly decreased in the treated pots when Compared to control 2. Significant increase was observed in moisture content, nitrogen, potassium, and pH, phosphorus in control 2 when compared to CD75.Soils supplemented with Aspergillus flavus showed spent that spent engine oil had no effect on the silt, sand, clay, moisture and nitrogen content while significant increased were observed in pH, potassium and phosphorus Contents of the soil due to spent engine oil contamination. Significant decreased Was was observed in carbon content of the soil.