Comparative Phytochemical Constituents and Antioxidant Activities of Hot and Cold Methanol Extract of Taminalia catappa Leaves.

Abstract

Comparative phytochemical constituents and antioxidant activities of hot and cold methanol extracts of T. Catappa leaves (TCLH and TCLC) were carried out using standard methods. The qualitative screening of TCLH revealed the presence of total phenols, flavonoids, tannins, saponins, alkaloids, reducing sugars, Phlobatannins, anthraquinones, glycosides and steroids while TCLC showed the presence of all aforementioned phytochemicals except reducing sugars and steroids. Both extracts exhibit percentage scavenging activities of 2, 2-diphenyl-1-picryl hydrazyl radicals (DPPH) in dose dependent manner with the highest percentage in 1000μg/mL (70.14 ± 0.06 and 50.30 ± 0.24 ) % and lowest at 125μg/mL (62.37 ± 0.41 and 44.31 ± 0.21) % for TCLC and TCLH respectively. The percentage inhibition of lipid peroxidation is significantly high in TCLH (84.14 ± 0.05) % when compared with TCLC (70.14 ± 0.06) % at the concentration of 1000ug/mL. Although, the % DPPH scavenging activities of both extracts are not comparable to the Gallic acid at 1000μg/mL (77.83 ± 0.06) % as well as other concentrations (500, 250 and 125μg/mL). However, TLCH exhibits better percentage inhibition of lipid peroxidation (84.14 ± 0.05) compared with Gallic acid (77.62 ± 0.50) % at 1000μg/mL. Therefore, TLCH and TCLC can further be implored in the management of oxidative-stress related diseases.