Isolation of multi-drug resistant Escherichia coli from urogenital samples of patients with pelvic inflammatory disease in Niger State.

Abstract

This study was conducted to determine the prevalence of multidrug resistant (MDR) Escherichia coli isolated from patients with PID attending nine hospitals in Niger State. A total of 1170 endocervical swabs (ECS) and urine samples were collected using sterile swap stick and sample containers. The samples were transported on ice pack to the Microbiology laboratory, Federal University of Technology, Minna for analysis.Screening for the presence of Escherichia coli was done using streak method of inoculation on Nutrient agar and Macconkey agar. Isolates of Escherichia coli were identified through Gram staining and other biochemical tests. Seventy two (22.5%) and 98 (24.5%) Escherichia coli isolates were obtained from both ECS and urine respectively. The antibiotic susceptibility profile of the isolates to ten (10) commonly prescribed antibiotics was determined using Kirby-Bauer disc diffusion method on Mueller-Hinton agar. The antibiogram showed that a total of 79 (34.6%) Escherichia coli isolates out of 228 multidrug resistant bacteria expressed multidrug resistant characteristics, and were resistant to more than three (3) classes of antibiotics. The multidrug resistant Escherichia coli exhibited 100% resistance to: Gentamicin in General Hospital Suleja; Cephalexin and Nalidixic acid in General Hospital Minna; Gentamicin and Streptomycin in General Hospital Kuta; Augmentin and Sulfamethoxazole in General Hospital Agaie; Cephalexin and Nalidixic acid in General Hospital Lapai; Augmentin in General Hospital Wushishi; Perfloxacin and Augmentin in General Hospital Nasko; followed by 92.9% resistance to Ofloxacin in General Hospital Bida and 80% resistance to Gentamicin and Cephalexin in General Hospital Kontagora. The results of this study confirmed the presence of multidrug resistant Escherichia coli in Niger State, hence there is a need for public health workers, to create awareness on the misuse of antibiotics, to prevent and curtail treatment failure due to antibiotic resistance.