Production of Amylase and Protease from Bacillus subtilis and Pseudomonas aeruginosa Isolated from Soil

Abstract

The study focused on the production of amylase and protease enzyme using bacteria isolated from soil. Soil samples were collected from Federal University of Technology Minna, Bosso campus using sterile dry polyethylene bag and kept at room temperature until use. Bacteria were isolated from the soil sample using cultural and biochemical tests. The bacteria isolated were screened for their ability to produce amylase and protease. Bacillus subtilis and Pseudomonas aeruginosa were identified to have the highest potential to produce the enzymes. The rates of biodegradation were determined using spectrophotometry. B. subtilis and P. aeruginosa were able to produce amylase and protease respectively. The effect of temperature (28 o C, 37 o C and 45 o C and pH (5, 7, 9) on the production of the enzymes by the isolates were determined. The optimum temperature and pH for the production of amylase and protease were 28 o C (1.988) and pH 5 (0.943) for B. subtilis while that of P. aeruginosa were 37 o C (1.092) and pH 7 respectively (0.042). The results of this study showed that Bacillus subtilis and Pseudomonas aeruginosa isolated from soil sample were good producers of protease and amylase and may be used for the large scale production of the enzymes.