PRODUCTION AND NUTRIENT ANALYSIS OF SINGLE CELL PROTEIN FROM AGROS-WASTE USING SACCHAROMYCESCEREVISIAE

Abstract

ABSTRACT This study was carried out to produce single cell protein (SCP) from pineapple peels (pp) and watermelon peels (wp) and a combination of the two samples (50/50 pp/wp) using Saccharomyces cerevisiae under submerged fermentation. The proximate, amino acid mineral and microbiological analyses were done using standard methods. The peels were collected, washed and dried in the oven at 50oC for 24 hours, ground and sieved using 0.05mm sieve. Forty grams of the pineapple and water melon peels each were separately hydrolysed with 1% HCl and sterilized by autoclaving at 121oC for 15min and filtered to get the substrates which were then added with mineral salt medium. The pH of the substrates was adjusted to 5.5 and the medium was inoculated with 2mL (5.0x103 cells) of Sacchromyces cerevisiae and incubated at 280C for 7days. The substrate was filtered and `the residue was dried in the oven at 500C for 24hours. The results of the proximate composition revealed that the crude protein (59%) and ash (7.2%) of the SCP (50/50 pp/wp) were significantly (p<0.05) higher than those of pp SCP and wp SCP while carbohydrate had the lowest value (20.11%). SCP (50/50 pp/wp) had significantly (p<0.05) high Na (2282 mg/100g), Ca (60172 mg/100g) and Mg (27312 mg/100g) contents while SCP (pp) had higher K (6000 mg/100g) and Fe (48 mg/100g) contents. The SCP from (pp) had comparative higher values for both essential and non- essential amino acids than either SCP (wp) or SCP (pp/wp). It is recommended that the use of SCP as food or in animal feed should be encouraged because of its high amount of protein, mineral and amino acids. FurtABSTRACT This study was carried out to produce single cell protein (SCP) from pineapple peels (pp) and watermelon peels (wp) and a combination of the two samples (50/50 pp/wp) using Saccharomyces cerevisiae under submerged fermentation. The proximate, amino acid mineral and microbiological analyses were done using standard methods. The peels were collected, washed and dried in the oven at 50oC for 24 hours, ground and sieved using 0.05mm sieve. Forty grams of the pineapple and water melon peels each were separately hydrolysed with 1% HCl and sterilized by autoclaving at 121oC for 15min and filtered to get the substrates which were then added with mineral salt medium. The pH of the substrates was adjusted to 5.5 and the medium was inoculated with 2mL (5.0x103 cells) of Sacchromyces cerevisiae and incubated at 280C for 7days. The substrate was filtered and `the residue was dried in the oven at 500C for 24hours. The results of the proximate composition revealed that the crude protein (59%) and ash (7.2%) of the SCP (50/50 pp/wp) were significantly (p<0.05) higher than those of pp SCP and wp SCP while carbohydrate had the lowest value (20.11%). SCP (50/50 pp/wp) had significantly (p<0.05) high Na (2282 mg/100g), Ca (60172 mg/100g) and Mg (27312 mg/100g) contents while SCP (pp) had higher K (6000 mg/100g) and Fe (48 mg/100g) contents. The SCP from (pp) had comparative higher values for both essential and non- essential amino acids than either SCP (wp) or SCP (pp/wp). It is recommended that the use of SCP as food or in animal feed should be encouraged because of its high amount of protein, mineral and amino acids. Furthermore, utilization of these agro-wastes will help in addressing environmental pollution caused by these wastes.ermore, utilization of these agro-wastes will help in addressing environmental pollution caused by these wastes.