Antibacterial potentials of silver nanoparticles synthesized from Calopogonium mucunoides leaves extract on some enteric bacteria.

Abstract

Silver nanoparticles (AgNPs) synthesized from Calopogonium mucunoides aqueous leaf extract and determination of the antibacterial activities against selected enteric bacteria were conducted in this study. The AgNPs were produced in an eco-friendly manner by combining a 1 mM silver nitrate solution with an aqueous C. mucunoides leaf extract under direct sunshine. UV-visible spectrophotometry, dynamic light scattering (DLS), and high-resolution transmission electron microscopy (HRTEM) were utilized to study the AgNPs (HRTEM). AgNPs were brown in colour and had a UV-Vis absorbance peak at 441– 445 nm. DLS uncovered monodispersed AgNPs with an 80.77 nm hydrodynamic diame- ter. A HRTEM image revealed that AgNPs with an average size of 7.5 nm had a spherical morphology. The antimicrobial susceptibility of colloidal AgNPs (50, 75, 100, and 150 μg/ mL) utilizing agar diffusion techniques against bacterial isolates (Pseudomonas aeruginosa strain DM1, Salmonella typhi strain T8, and Escherichia coli Acj 213) revealed inhibition zones ranging from 16.70 to 21.00 mm. For three bacterial isolates, the lowest inhibitory con- centrations were 37.50, 37.50, and 9.38 μg/mL, and the minimum bactericidal values were 75, 75, and 37.5 μg/mL, respectively. The outcomes of this study indicate that the aqueous leaf extract of C. mucunoides can be used to synthesize AgNPs with a considerable anti- bacterial impact against clinical isolates of P. aeruginosa strain DM1, S. typhi strain T8, and E. coli Acj 213. To assure the safety of biogenic AgNPs for the development of novel antimicrobial drugs, additional research is needed to assess their toxicity. SCIENCE FORUM (JOURNAL OF PURE AND APPLIED SCIENCES) (2023) 429