Gastroprotective Activity of N- hexane and Chloroform Fractions of Sesasum radiatum leaf on Aspirin induced Ulceration in Rat.

Abstract

Gastric ulcer is one of the most common gastrointestinal disorders, which causes a high rate of morbidity all over the world. Available drugs for ulcer treatment are associated with several side effects, thus the need to search for alternative treatment using plants and vegetable which are readily available and safer. The effect of n-hexane and chloroform fractions of Sesamum radiatum leaf extract on aspirin inducedulceration in albino rats were investigated. Ulcerative index, alkaline phosphatase (ALP), aspartate amino transferase (AST), alanine amino transferase (ALT), superoxide dismutase (SOD), catalase (CAT), Malondialdehyde (MDA) and the extent of inhibition of lipid peroxidation were determined using standard methods. Acute toxicity study revealed that the fractions were nontoxic up to a dose of 5000mg/kg bodyweight. Animals administered with 100 and 500mg/kg bodyweight of n-hexane fraction exhibit inhibition of ulceration of 33.33% and 50.00% while those of chloroform fractions showed 50.00% and 66.0% inhibition respectively compared to the 0% of the untreated group. AST, ALT, ALP and MDA Level of the ulcer induced rats treated with both fractions were significantly (p<0.05) reduced in a dose dependent manner. The SOD and CAT activities of Sesamum radiatum fractions treated groups showed significant increase as compared with untreated and toxic group. It can therefore be suggested that the n-hexane and chloroform fractions of S. radiatum may possess considerable therapeutic potential in the management of gastric ulcer.