Optimizing Deproteinization of Chitin Using Response Surface Methodology

Abstract

The physicochemical parameter which influences the application of chitin in various fields is strongly linked to the source and the conditions of the chitin production. The condition of chitin can be controlled by manipulating solution conditions (temperature, concentration, time) and optimization of deproteinization processes. The Callinectes pallidus waste was pre-treated, bleached, decolourized and demineralised which significantly affect chitin quality and yield. The design of experiments (DOE) via central composite design (CCD) of response surface methodology (RSM) was used to optimize the process parameters of deproteinization. The experimental results indicates that the CSW/chitin had 5.24/4 % moisture, 59.31/0.7 % ash, 6.75/0.95 % lipid, 15.75/2.05 % crude protein, 44.17/8.98 g/Kg mineral, 374 m2 /g specific surface area and 0.25 cm3/g pecific pore volume respectively. The predicted values of the response was further validated and suggested the optimal conditions for deproteinization of chitin were 70 oC, 4 h, and 4.47 % of NaOH with percentage deproteinization of 92.97 % and 91.08 % for actual and predicted value respectively