Modelling of Serpentine Continuous Flow Polymerase Chain Reaction Microfluidics

Abstract

The continuous flow Polymerase Chain Reaction (PCR) microfluidics DNA amplification device is a recent discovery aimed at eliminating the cyclic hold experienced while using the alternative stationary device. The Application of Computational Fluid Dynamics is increasingly growing and can help achieve optimal designs before actual fabrication. This paper presents a CFD modelling of a continuous flow serpentine PCR device with narrow and wider channels. There are two temperature regions at 950 C and 600 C for denaturation and annealing respectively. Extension is achieved along the middle of the channel at 720 C owing to temperature gradient. The model require a pressure of 42.6KPa for a 30 cycle amplification.