Inhibitory activity of xanthine oxidase by fractions Crateva adansonii

Abstract

Objective: To study the inhibitory effect of various extracts from Crateva adansonii (C.adansonii) used traditionally against several inflammatory diseases such as rheumatism, arthritis, and gout, was investigated on purified bovine milk xanthine oxidase (XO) activity. Methods: Xenaznytmhien ien hoixbiidtiaosne winash idbeitteorrmy iancetdi vbiyty m weaass uarsinsga ytehde isnpcerceatrsoep ihno atobmsoertbraicnaclel ya t and the degree of with uric acid formation. 295 nm associated xanthine as the substrate. ERnezsyumltes :k inetics was carried out using Lineweaver-Burk plots using Among the fractions tested, the chloroform fraction exhibited highest potency (IC50 20.2依1.6 毺g/mL) followed by the petroleum ether (IC50 30.1依2.2 毺g/mL), ethyl acetate (IC50 43.9依1.4 毺g/mL) and residual (IC50 98.0依3.3 毺g/mL) fractions. The IC50 value of allopurinol used, as the standard was 5.7依0.3 毺g/mL. Conclusions: Enzyme inhibition mtheec thhaenriaspme uintidci cuastee do ft htahte tshee pmlaondtes omf ainy hbibei tdioune wtoa st hoef ao bmseixrevded t yXpaen. tOhuinr ef inoxdiidnagsse s uinghgeibsit tithonat, tihne praerbtyic suulaprp, ogrotiuntg. their use in traditional folk medicine against inflammatory-related diseases,